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Intracellular activation of nitrofurantoin yields a radical anion that subsequently initiates Nrf2-dependent induction of glutamate-cysteine ligase and consequently a rise in glutathione

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Detection of deposited TCC (C5bC9) was done using a biotinylated mouse monoclonal antibody specific for a C9 neoepitope exposed upon C5b binding (Diatec Monoclonals, DIA 011-01) (diluted 1:5000 in PBS/T/S) together with AP-conjugated streptavidin (diluted 1:5000 in PBS/T/S) (Cytiva Life Sciences) and visualized by addition of AP substrate (Sigma-Aldrich)

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