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Step 2 is to determine at that water activity, what is the most likely mode of failure

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Storage and Handling Guidelines Proper storage is essential for both types: Store at room temperature unless otherwise indicated Do not use if solution appears cloudy or contains particles Use sterile laboratory tools and aseptic technique Discard opened sterile water immediately after use Follow manufacturer guidance for bacteriostatic water reuse timelines Conclusion Bacteriostatic water and sterile water may look identical, but their roles in research and pharmaceutical environments differ significantly

Cages were exchanged for new sanitized cages filled with irradiated bedding every 2 weeks