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Microinjection and EV-treatment comparison Halo-Rac1 G12V protein was diluted to 300 g/ml in a buffer containing 10 mM Tris-HCl, pH7.5, 150 mM NaCl, 5 mM MgCl 2 , and 6.25 mg/ml of 0.22 m-filtered Rhodamine B isothiocyanate-Dextran (Sigma, R9379)

As GSH and GSSG will react spontaneously with each other and with other thiol and disulfide compounds of the cell, in the thioldisulfide exchange reaction, the ratio of GSH and GSSG reflects the general redox properties of the cell