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Validation criteria should extend beyond basic analytical performance (sensitivity, specificity, and reproducibility) to include clinical practicality metrics, such as compatibility with routine clinical sample processing workflows, rapid matrix application protocols, and scalability for large-scale clinical cohorts

doi: 10.1016/0190-9622(90)70017-c

This matters because Melanotan-2 research protocols often involve dose titration in 100250mcg steps, and imprecise dosing at this scale skews results

Recently, the introduction of artificial intelligence (AI) and machine learning (ML) technologies has injected new impetus into matrix study and development