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Closer immunophenotyping of the cellular content of the BALF and lung tissue by flow cytometry showed that AA-exposed mice had significantly greater levels of eosinophilia in both compartments compared to PBS and melatonin-only controls ( Figures 1G, H )

Subsequently, 20 l of buffer containing 10 mM Tris-HCl (pH 7.5), 300 mM sucrose and 1.0 mM EDTA was added to the glass tube, which was then sealed and incubated at 45 C for 8 min (prehydration)

[DOI] [PubMed] [Google Scholar] 16.Huang Z., Liu Y., Qi G., Brand D., Zheng S.G