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Hence, we expected to observe an increase in serum concentrations of 17-estradiol in GS

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S 43 then carries out a nucleophilic attack on S 48 , leading to the formation of the redox-active disulfide bridge between Cys 43 and Cys 48 , followed by the cleavage of the covalent bond between S 48 and flavin atom C-4a, producing the reduced FADH anion with a negative charge on atom N-1
