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To determine nonspecific signals, the primary antibody incubation step was omitted

A critical limitation to the expression of the otherwise numerous therapeutic effects of catechins is their insufficient in vitro stability

Khanna-Chopra and selote 72 in wheat, Ozkur et al

The expression of target genes was analyzed via quantitative real-time (RT) PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)
