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Subsequently, samples were sonicated to ensure complete disruption of all cells using a sonication bath (Transsonic 460, Elma Sonic, Singen, Germany) for 5 min at the highest frequency on ice

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[DOI] [PMC free article] [PubMed] [Google Scholar] 183.Vasileska A., Rechkoska G

To lay a basis for further mechanistic investigations we performed a genome scale transcriptional profiling of the Arabidopsis thaliana response to an exogenous treatment with allyl-isothiocyanate (allyl-ITC) at three time points: 30 min, 1 h and 9 h