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Insulin resistance index (HOMA-IR) was calculated by the formula of fasting insulin (FIns, U/ml) fasting blood glucose (FBG, mmol/L)/22.5 as described in the previously published study ( 2.5 Western blot analysis Proteins were extracted from ovarian tissues, and their concentrations were determined using a bicinchoninic acid (BCA) protein quantification kit (Beyotime Biotechnology Inc., Shanghai, China)

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