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(B) The western blots of three independent experiments have been quantified using GelQuant.net software and the fold change in the protein expression levels of eIF4A1 was calculated by normalizing them against bands of -tubulin

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Glutamate is converted to -KG both via GLUD1, generating glutamate-derived nitrogen as ammonia, and via aminotransferases, which transfer nitrogen from glutamate to -KG to produce other NEAAs