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To calculate the reproducibility in 5-km times, the following was done: Reproducibility was calculated by obtaining the square root of the mean square error obtained from a repeated measures analysis of variance obtained from the two 5-km baseline time trials that were performed in a one week period

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Next day, the sections were washed three times in PBS followed by incubation with Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11008, Invitrogen) and Alexa Fluor 594 anti-mouse CD68 Antibody (137020, BioLegend, USA) or Alexa Fluor 594 anti-mouse Ly-6G Antibody (127636, BioLegend, USA) for 1 h at room temperature in the dark followed by washing and mounting

Available online: 17.Kather S., Grtzner N., Kook P.H., Dengler F., Heilmann R.M