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As an alternative to column purification, a protocol describing batch purifications is described in Note 15
Hickie I, Lloyd A, Hadzi-Pavlovic D, Parker G, Bird K, Wakefield D
Inflammation haptocorrin levels can increase due to granulocyte activation or as an acute phase response Decreased clearance: Renal failure decreased clearance of transcobalaminB12 complexes Mechanism: Most elevated B12 is not free or biologically active , but rather bound to haptocorrin , which has a long half-life and is increased in certain diseases
