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Interestingly, although previous evidence has suggested that AD patients generally demonstrate progressive deterioration in brain perfusion and clinical symptoms, a recent SPECT study depicted an increase in the perfusion of the precuneus in AD patients after the administration of ALC, along with a lack of further deterioration of cognitive and neuropsychiatric symptoms [52]

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In the substrate oxidation stress test, the cells were prepared as above prior to the assay, and the cells were subjected to (in sequence, in final concentrations): (1) 0, 4 mM l-glutamine (Seahorse Bioscience, Billerica, MA, USA), 40 M Etomoxir (11969, Cayman Chemical, Ann Arbor, USA) or 3 M BPTES ((bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide)