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After microscopic examination, oocytes at metaphase II stages (MII, with the polar body extrusion) were collected and cultured in 500 l of fertilization droplets as SOF medium supplemented with 5% fetal bovine serum, 2 mM sodium pyruvate, 10 g/ml heparin sodium and 1 mM caffeine in a CO 2 incubator (38.5C, 90%N 2 , 5%CO 2 and 5%O 2 ) (32)

Study materials Vitamin C (VIT C) capsules, liposomal vitamin C capsules (LV-VIT C, LipoVantage , Specnova, LLC, Tyson Corner, VA, USA) and placebo (PLA, maltodextrin) were acquired from Molecules Food Solutions Pvt Ltd, Kerala, India
Glutathione infusions, on the other hand, take a targeted approach by fighting oxidative stress and inflammation directly

A review and novel theoretical model of how negative emotions influence inflammation: the critical role of emotion regulation
