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Step 2: 5, 10-methylenetetrahydrofolate is converted into methylfolate (5-MTHF) by the enzyme methylenetetrahydrafolate reductase (MTHFR) with the aid of NADH, B2 and ATP

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Standardization process for detection technologies: international unified standards for metabolite quantitative detection must be formulated, encompassing preparation of standard reference materials, analytical methods for detection instrumentation, and operational protocols for quality control, to enhance comparability among results from different studies (166)

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