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doi: 10.1016/s1097-2765(00)80098-2

169 , e70146 (2025)
Iron overload promotes intervertebral disc degeneration via inducing oxidative stress and ferroptosis in endplate chondrocytes

Frozen hepatocytes were thawed on ice and resuspended in 0.1 M phosphate buffer, pH 7.5, containing 2 mM tris(2-carboxyethyl)phosphine (TCEP) to reduce disulfide bonds linking thiols to protein sulfhydryl groups or to each other
