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To compare the expression of these genes in response to H 2 O 2 , we measured by qPCR the transcriptional level of each one in the pneumococcal R801strain (derivate of the R6 strain) [31] exposed at 20 mM H 2 O 2 for 30 min, and referred to gyrB as internal control [26]

In the IR group, an atraumatic microclamp was applied to the infrarenal aorta for 45 min, followed by a 120 min reperfusion period after clamp removal

This procedure was performed on each of the segments, and an optimal n was chosen for each of the following: autoantibodies ( n = 5), SARS-CoV-2 antibodies ( n = 3), non-SARS-CoV-2 viral antibodies ( n = 32), plasma proteomics ( n = 18) and flow cytometry ( n = 21)