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Samples (5 l) were eluted with a linear gradient from 95:5 (A:B) to 0:100 (A:B) over 10 min, then held at 0:100 (A:B) for 1 min

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The binding pose of the naphthylsulfonic acid core of the analogues was constrained to the leucine pocket, imitating the binding mode of 32 , whilst the substituted benzyl group was left unconstrained in the phenylalanine pocket

However, its direct impact on intrinsic quality parameters, such as the brown rice rate, milled rice rate, chalky grain rate, chalkiness degree, protein content, and amylose content remain limited, as these traits are largely influenced by genetic factors, environmental conditions, and cultivation practices