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The large number of IGFBPs, IGFBP-rPs as well as other carrier proteins affect bioavailability of IGF-1 in circulation and reduces significantly the levels of immunoreactive IGF-1 in serum samples, requiring multiple pre-treatment steps that reduce reproducibility and complicates interpretation of IGF-1 assay results

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CYP enzymes including CYP2D6 and CYP2C9 are part of the cytochrome P450 family primarily involved in xenobiotic metabolism
UV is generated artificially by mercury lamps, which emit an electromagnetic radiation spectrum ranging from 100 to 400 nm
