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Digestion was completed by heating the samples to 90 C for 20 min, followed by dilution into an equal volume of hydrogen peroxide (30% Aristar BDH) and incubation for ~30 min period, allowing for effervescence to stop

Both DHFR and TYMS can autoregulate their synthesis by binding to their own mRNA, repressing translation [91,92]

186 , 19 (2019) Chen, X., Wang, X., Cao, G., Wu, Y., Luo, H., Ji, Z., Shen, C., Huo, D., Hou, C.: Colorimetric and fluorescent dual-identification of glutathione based on its inhibition on the 3D ball-flower shaped Cu-hemin-MOFs peroxidase-like activity
