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Culture-Independent Approach Besides that, different approaches have been developed to study the microbiome, including clone libraries, terminal restriction fragment length polymorphism (T-RFLP), quantitative PCR (qPCR), transcriptome microarrays, as well as high throughput sequencing technologies (Illumina sequencing and 454 pyrosequencing)

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Sugden, 2007)

149 Under specific conditions, ROS can bypass these pathways to induce pyroptosis, driven by inflammasome activation (e.g., NLRP3) in response to PAMPs/DAMPs, the predominance of inflammatory caspases (caspase-1/4/5/11) over caspase-8 or RIPK3, and cell-specific mechanisms prioritizing cytokine release (IL-1/IL-18) in immune cells like macrophages