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doi: 10.1016/j.jaim.2017.11.003 94 KhalifaH
The cells were replenished with RPMI Cell Differentiation Medium without ROCK inhibitor the next day and cultured for 5 days with daily medium changes
Subsequently, 300 l of cellular extraction buffer (80% methanol, containing 2 M d27 myristic acid) were added to the cells and incubated for 23 min on ice before scraping the cells
Homozygosity for mutant NF1 alleles has not been reported, probably because it is a lethal condition
