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EnvA pseudotyped rabies-G-GFP was injected into AgRP neuron terminal areas (PVH example) in Agrp -IRES-Cre mice that were previously injected with AAV-FLEX-TVA-mCherry in the arcuate

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This accelerates ATP synthesis and induces hyperpolarization of the mitochondrial membrane potential, which impairs electron transfer at Complex III

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While CpG dinucleotide methylation remains stable in many contexts, other forms of methylation, such as CH methylation, play crucial roles during early brain development (Ma et al., 2017)

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