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Before the experiment, a working solution of dihydroxyrhodamine (5 M) is diluted from the stock, kept on ice, and protected from light
At 20 years, levels average around 200 ng/ml, while studies document concentrations drop to approximately 80 ng/ml by age 60[2]
It is crucial to carefully consider the chosen laboratory parameters regarding differentiating the influence of iron on bone, as each may imply different conditions
