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Per 6-well, 8 l of lipofectamine 2000 (Thermo Fisher, 11668019) were used together with 1.2 g of pLKO.1 plasmid expressing the shRNA against the protein of interest, 0.65 g of pVSV.G, and 0.65 g psPAX2

ORMDL orosomucoid-like proteins are degraded by free-cholesterol-loading-induced autophagy

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