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After incubation the cerebrovascular fractions were washed three times with PBS and collected in 500 l of lysis buffer
The resulting cells were harvested through centrifugation at 800 rpm for 5 min and then washed twice with ice-cold phosphate-buffered saline (PBS)
In San Cristbal de La Laguna, the most popular natural swimming pools are those at Punta Hidalgo
[DOI] [PMC free article] [PubMed] [Google Scholar] 51.Callaghan, B
