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10% (v/v) glycerol) containing 2% N -dodecyl -d-maltoside for a 20-min incubation on ice, followed by centrifugation at 20,000 g for 10 min

[DOI] [PMC free article] [PubMed] [Google Scholar] 167.Marwick JA, Kirkham PA, Stevenson CS, Danahay H, Giddings J, Butler K, et al

doi: 10.1016/j.pharmthera.2016.07.010

Collectively, this eventually leads to the cleavage and fragmentation of the protein backbone, yielding carbonyl and amide fragments (66)
