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The mobile phase consisted of acetonitrile and 10 mM ammonium acetate with 0.2% formic acid in the water, and was applied using a gradient elution as follows: 0 min90% ACN, 6 min80% ACN, 7.3 min35% ACN, 8 min35% ACN, 8.3 min90% ACN, and 10 min90% ACN

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The diet and medication may need to be adjusted based on blood test levels