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The oxygen consumption (VO 2 ) and carbon dioxide production (VCO 2 ) were measured after calibration

Cell death was visualized by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) according to the manufacturers instructions (In situ cell death detection kit, TMR red

The platform demonstrates tumor specificity, durable degradation kinetics, and potent antitumor activity with minimal off-target effects

In the field of genomics, by analyzing key information such as individual-specific target variations, mutation loads, complex mutation characteristics, and tumor-specific antigens, a solid theoretical foundation has been laid for the three pillars of precision medicinetargeted therapy, immune checkpoint inhibitors, and personalized anticancer vaccines (Miao and Van Allen, 2016