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Possible solution A: Change expression system according to literature on protein of interest and on bacterial expression systems, or use a nonbacterial expression system, for example, in vitro translation, as long as sufficient GST fusion protein for pull-down can be obtained

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It is a reactive sulfur species (RSS), which causes a redox reaction with thiol groups in glutathione and proteins and is considered to be important for its biological activity [10, 11]

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