Lipid Peroxidation in Hepatic Fibrosis
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As a stimulator of ODC, Asn likely promotes TJs stability through increased polyamine synthesis, which is known to be essential for TJs protein synthesis and maintenance (Chen et al
Cell pellets were resuspended in 700 L of ice-cold co-IP buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Nonidet P-40,1 mM EDTA pH 8.0, 1 mM PMSF, AEBSF, Serva protease cocktail and a Complete Mini EDTA-free cocktail of protease inhibitors which was freshly added) and glass beads
