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Inhalation 5.4.3.3.2

However, since cell counting is only a rough proxy to assess cytotoxicity and a higher dose escalation is necessary for CC 50 determination, we further included careful assessment of growth kinetics by live cell imaging, measurement of mitochondrial metabolic activity by MTT assay as well as cellular ATP levels by CellTiter-Glo (CTG, see Material and Methods section for details)

Through this network-oriented reevaluation, we aim to provide a unifying theoretical perspective that organizes fragmented observations, highlights key regulatory hubs, and generates testable predictions to guide future basic research and the development of potential clinical intervention strategies