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All experimental procedures were carried out at the Gazi University Animal Laboratory following ethical guidelines approved by the Gazi University Experimental Animal Ethics Committee (G..ET-24.075)

Supplied in capsule format for controlled laboratory workflows, 5-Amino-1MQ supports reproducible experimental design while remaining strictly designated for research use only

Laboratory animals were housed in polycarbonate (PC) cages under conventional laboratory conditions at 2024C, relative humidity of 4070%, and noise level of 60 dB

If tolerated, the documented approach is to stay at the current step for the full interval before the next scheduled escalation not to leapfrog steps