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The blots were washed three times for 30 min with TBST, after which they were incubated with conjugated anti-rabbit secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA, United States) diluted with blocking buffer (without 0.02% sodium azide) for 4 h at room temperature

If weight change is a goal, ask a clinician about evidence-based nutrition, activity, sleep, medication, and metabolic factors

In this study, we inactivated GSTA4 in HCT116 human colon cancer cells and investigated the effect of GSTA4 on cancer cell proliferation and chemoresistance