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Twenty L of each sample was transferred to a Nunc 96-Well Polypropylene MicroWell Plate (Thermo Scientific Nunc, Roskilde, Denmark) and mixed with 180 L of master mix

The final confirmation usually comes from examining the tissue under a microscope after surgery

Due to its relevance in metabolic and biochemical pathway research, 5-Amino-1MQ remains a continued subject of interest in experimental models examining cellular regulation, energy-associated biological processes, and molecular signalling behaviour under controlled laboratory conditions