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Description
Research on oxidative peptide damage recommends working under argon or nitrogen atmosphere for sensitive sequences

Experimental Section Synthesis N -methylpyrrolidone (NMP) was used to remove the Fmoc group, and coupling was carried out using 0.3 M Fmoc-amino acid in 0.3 M OxymaPure in DMF added in 68 equivalent excess and activated by 68 equivalents of DIC (3 M solution in DMF) and 34 equivalents of collidine (3 M solution in DMF)

Measurement of ROS by DCFH2-DA Cells were seeded in 24-well plates at a density of 5 10 cells per well and cultured in fresh medium for 12 h

PMID: 34424319
