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Mol Cell Biol (2020) 40
After release of the protoplasts by soft shaking, the suspension was overlaid with MMM solution (5 mM MES pH 5.8, 15 mM MgCl 2 , 467 mM mannitol), and protoplasts counted in a Rosenthal chamber
doi: 10.1016/j.devcel.2019.04.029 105 PloumiCDaskalakiITavernarakisN
George Clooney: A Biography
