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The diluted supernatant was then centrifuged at 100,000 x g and the supernatant was loaded onto a Ni-NTA column pre-equilibrated with buffer A (20 mM HEPES, pH 8, 100 mM NaCl, 10 mM -mercaptoethanol, 0.1 mM EDTA, and 0.1 M EGTA)

Moreover, complete loss of H3K27 trimethylation was significantly more frequent in the Triton subtype compared with spindle and pleomorphic cell subtype malignant peripheral nerve sheath tumors, which is in accordance with literature data showing that MPNSTs demonstrating rhabdomyoblastic differentiation are particularly aggressive and associated with poor prognosis