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Additionally, a microfluidic microplate-based immunoassay offers rapid detection with reduced antibody use compared with traditional ELISA, reaching limits as low as 6.25 10 3 U/L

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The 4-PBA group showed mild sinusoidal congestion with focal hepatocyte necrosis as manifested by nuclear pyknosis, karyorrhexis, and karyolysis with cytoplasmic eosinophilia (Figure 8A), yet it significantly attenuated histopathological scoring compared to MTX-group (Figures 8BD)

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