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The supernatant was passed over Ni-NTA agarose (Qiagen) and the protein was eluted in elution buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 250 mM imidazole and 1 mM DTT)

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From a technological standpoint, high equipment costs, long processing times, and elevated energy demands remain critical barriers to the adoption of advanced extraction methods, particularly in developing regions with limited research infrastructure