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Incubate with 1.5% milk at room temperature for 1 h and wash overnight with 0.3% Triton-100 at 4 C

Journal of Pediatric and Adolescent Gynecology , 30 (5), 520534
Additives, only the manufacturers might know about how long they work in various situations

Guillot TS, Shepherd KR, Richardson JR, Wang MZ, Li Y, Emson PC, et al
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