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For the 2p-FLIM ensemble machine-learning analysis, we first took GFP and Nile Red images, applied the same image process protocol described in Section 2.5 to identify the particles with both Nile Red and GFP signals, and used those DHS-3::GPF-positive and VIT-2::GPF-positive Nile Red particles as the ground-truth for non-polar lipid-dominant and polar lipid-dominant particles respectively

Shields and Shields 2015

