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Remove the nasal spray from the packaging and lay the person on their back Hold the nasal spray with your thumb on the bottom of the plunger and your middle fingers on either side of the nozzle Tilt the persons head back and insert the nozzle into one nostril until your fingers are against their nose Press the plunger firmly to give the dose and then remove from the persons nose If the person does not respond in 2-3 minutes, give a second dose from a new nasal spray (if available) Instructional Videos If you or someone you know is at risk for opioid overdose, it is important to have Narcan available

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Statistical analysis All experimental data are expressed as mean standard deviation ( n 3) and were analyzed using Students t test to determine the statistical differences between 2 groups