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Immunoblotting The dishes were washed 2 with ice-cold PBS, lysed in 1 Laemli sample buffer, and subjected to standard SDS-PAGE using separate 4%12% Bis-Tris gels, after which the proteins were overnight transferred at 4C to polyvinylidene fluoride membranes

It is estimated that by 2050, there will be 1.31 billion people living with diabetes globally

The requirement for general anesthesia excludes some patients and adds cost and complexity to the diagnostic process (Chapron et al., 2003)

Additionally, AChE and ADA may be contributed to clinical signs of apathy because of their participation in neuromodulation and neurotransmission (264)