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Other as yet uncharacterized proteins may also have DHAR activity (Morell et al
ELISA assays showed that BV2-metformin-loaded PLGA lowered pro-inflammatory markers (TNF-, IL-6) and boosted anti-inflammatory markers (IL-4, IL-10) in both the brain tissue and blood samples 19
Twenty-four hours post-injection, the mice were euthanized and perfused with cold phosphate-buffered saline (PBS) to eliminate circulating blood and unbound dyes
