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The methods used by the CDC for analyzing testosterone in NHANES serum samples can be found at To control for the effects of baseline mortality risk factors, the following covariates were included in the present analyses: (i) age, (ii) race/ethnicity, (iii) body mass index, and (iv) education as a proxy for socioeconomic status

They use HPLC purity verification and mass spectrometry for identity confirmation

Malabsorption Disorders: Conditions such as pernicious anemia, Crohns disease, celiac disease, or a history of gastric bypass surgery can hinder B12 absorption
