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And compared to control cells, the IBDV titers of recombinant GSS-L, GSS-M, and GSS-H cell lines were increased by 1.74 0.50, 7.19 0.93, and 0.96 0.32-fold, respectively (Fig
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[DOI] [PMC free article] [PubMed] [Google Scholar] 175.Mai, C.-T

According to Warner et al

Its good practice to wash your column with a couple column volumes of binding buffer to remove any unbound proteins
