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CRNs exhibit diverse mechanisms, applications, and advantages that enhance their performance in targeted and controlled drug delivery (Table 1)

The increased levels of cGMP alter transmembrane calcium ion flux, resulting in cavernosal smooth muscle relaxation, dilatation of cavernosal and helicine arteries and engorgement of lacunar spaces

Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere