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found that measurements of parameters such as tumor necrosis factor alpha (TNF-), interleukin 6 (IL-6), and interleukin 10 (IL-10) were not significantly changed after a course of IF (3)

doi: 10.1016/s0735-1097(01)01121-4
For negative controls, the staining solution included 5 mM serine (Sigma-Aldrich, S4311) and 10 mM sodium borate (Sigma-Aldrich, 71997)

& Krediet, R
