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Serum samples in different groups were incubated with detection reagent for 30 min at ambient temperature, while absorbance values were measured at 560 nm (M200 Pro, Tecan, Switzerland)

Anti-Inflammatory and Antioxidant Effects: Investigate suppression of pro-inflammatory cytokines (e.g., IL-6, TNF-) and enhancement of superoxide dismutase (SOD) and other antioxidant pathways

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